SM Agar (1 Liter)
| Peptone | 10 grams |
| Yeast Extract | 1 gram |
| Glucose | 10 grams |
| KH2PO4 | 1.9 grams |
| K2HPO4 | 1.3 grams |
| MgSO4anhydrous | .49 grams |
| Agar | 17 grams |
** SM Broth is without Agar
HL5 Medium (1 Liter)
| Peptone | 14 grams |
| Yeast Extract | 7 grams |
| KH2PO4 | 0.5 grams |
| Na2HPO4 | 0.5 grams |
| Glucose | 31.5 grams |
LB-Broth Miller (1Liter)
| Tryptone | 10 grams |
| Yeast Extract | 5 grams |
| NaCl | 10 grams |
| Final PH | 7.0 ± 0.2 |
KK2 buffer (1 liter)
| KH2PO4 | 2.2 grams |
| K2HPO4 | .7 grams |
Starving Buffer (100 mls)
| Na2HPO4 | 1.78 grams |
| KH2PO4 | 9.9 grams |
**Water Agar Plates are 1% for example for 300ml add 3 grams of Agar
Starving Plates (1 Liter)
| Starving Buffer | 20 mls |
| H2O | 980 mls |
| Agar | 20 grams |
Hay Plates (1 Liter)
| KH2PO4 | 1.5 grams |
| Na2HPO4 | .62 grams |
| Agar | 15 grams |
TBE (1X)
| Tris Base | 216 grams |
| Boric Acid | 110 grams |
| Na2EDTA | 18.6 grams |
| ddH2O | 20 Liters |
LP Agar (1 Liter)
| Lactose | 1 gram |
| Bactopeoptone | 1 gram |
| Agar | 15 grams |
SS ( 1 Liter)
| NaCl | 0.6 grams |
| KCL | 0.75 grams |
| CaCl2 | 0.3 grams |
The Queller/Strassmann Lab 